en عمومى General پژوهشي Research <p><b>Background:</b> Since hand hygiene is a key strategy for infection control, considering appropriate instrument for evaluating health care providers&rsquo; hand hygiene is essential. In this study, translation process and evaluating reliability and validity of hand hygiene questionnaire (HHQ) is described.&nbsp;</p> <div><b>Methods:</b> HHQ was translated into Persian with combined method. In this cross-sectional study, the questionnaire was given to 60 nursing students in sixth semester. The internal consistency, test-re-test reliability, convergent construct validity of the questionnaire were measured. The data were analyzed by SPSS software, 16th version.&nbsp;</div> <div><b>Results:</b> The cronbach&rsquo;s alpha of HHQ was 0.80. Intra class correlation coefficient ranged from 0.70-0.85 and standard error of measurement was low. There were no significant statistical differences between test and retest. Also convergent construct validity of HHQ was optimal.&nbsp;</div> <div><b>Conclusion:</b> The results indicated that validity and reliability of HHQ-Persian version were appropriate and satisfactory. It can be used for nursing students however, for other health care providers further evaluation is essential.</div> Hand hygiene questionnaire, Hand hygiene knowledge, Hand hygiene belief, Hand hygiene practice, Instrument validity and reliability 23 28 http://jccnc.iums.ac.ir/browse.php?a_code=A-10-1-4&slc_lang=en&sid=1 Tahereh Najafi Ghezeljeh 10031947532846002783 10031947532846002783 Yes School of Nursing and Midwifery, Iran University of Medical Sciences, Tehran, Iran. Maryam Khosravi 10031947532846002784 10031947532846002784 No Tehran University of Medical Sciences Tahereh Tolouei Pourlanjarani 10031947532846002785 10031947532846002785 No Iran University of Medical Sciences